Part:BBa_K1159312
N-terminal Strep-tag-II in RFC[25] N-Part
This parts codes for a N-terminal Strep-tag-II flanked by RFC[25] N-part to allow C-terminal fusions. Strep-tag II is originally designed by Prof. Arne Skerra from TU Munich.
Usage and Biology
The so called N-terminal encoded Strep-Tag-II flanked by RFC[25] N-part consists of the following amino acid sequence: Trp-Ser-His-Pro-Gln-Phe-Glu-Lys. The iGEM Team of TU Munich 2013 used the Streptag to purify several recombinant proteins, which have been expressed in E. coli, by affinity chromatography. An extraordinary benefit of the Strep-tag is its small size and the fact that it is almost inert to biochemical reactions, except its binding affinity to Biotin-labelled beads. Further on Strep-Tag antibodies with enzymatic or reporter genes can be used to target the Strep-Tag. Some applicational examples are Western-Blots, affinity protein purification and several assays.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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