Device

Part:BBa_K1159308

Designed by: Dong-Jiunn Jeffery TRUONG   Group: iGEM13_TU-Munich   (2013-09-14)

Neomycin phosphotransferase II (nptII) plant expression cassette for G418 resistance

This part contains the plant expression cassette for the Neomycin Phosphotransferase II (nptII) responsible for G418 resistance. NptII is driven by NOS promoter and terminator. This part is flanked by RFC[10] pre- and suffix.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 251
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1185
    Illegal BamHI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1002
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 851
    Illegal SapI.rc site found at 1061


[edit]
Categories
//chassis/eukaryote/ppatens
Parameters
None