Part:BBa_K1159214
Membrane-anchored SpyTag with secretory nLuc_SpyCatcher reporter fusion translational unit
This part encodes a translational unit consisting two fusion proteins, a Membrane-anchored SpyTag and a secretory nLuc_SpyCatcher (IgKappa-SigP_nLuc_SpyCatcher) reporter fusion to show if post-translational protein fusion like reaction happens between the membrane-localized tag protein and the secretory fusion protein containing N-terminal nLuc and C-terminal SpyCatcher Luciferase. As a translational unit both fusion proteins are seperated by a polioviral IRES to allow bicistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1384
Illegal XhoI site found at 1880 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1148
Illegal AgeI site found at 1503 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 159
Illegal BsaI.rc site found at 1077
Illegal SapI.rc site found at 1581
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