Part:BBa_K1159212

Membrane-anchored SpyCatcher with secretory SpyTag_nLuc reporter fusion translational unit

This part encodes a translational unit consisting two fusion proteins, a Membrane-anchored SpyCatcher and a secretory SpyTag_nLuc (IgKappa-SigP_SpyTag_nLuc) reporter fusion to show if post-translational protein fusion like reaction happens between the membrane-localized catcher protein and the secretory fusion protein containing N-terminal SpyTag and C-terminal NanoLuc Luciferase. As a translational unit both fusion proteins are seperated by a polioviral IRES to allow bicistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1684
  Illegal XhoI site found at 2225
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1448
  Illegal AgeI site found at 1803
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 459
  Illegal BsaI.rc site found at 1377
  Illegal SapI.rc site found at 1881