Translational_Unit

Part:BBa_K1159116

Designed by: Dong-Jiunn Jeffery TRUONG   Group: iGEM13_TU-Munich   (2013-09-16)


Eukaryotic light-switchable TEV Protease translational unit (PhyB/PIF6 based) with downstream IRES

This part encodes a light-switchable TEV Protease system consisting of two fusion proteins (BBa_K1159106 and BBa_K1159108) seperated by an polioviral IRES BBa_K1159300 which allows polycistronic expression in eukaryotic systems. You can easily express a third protein by simply clone your desired protein in the suffix of this part because this part ends with a polioviral IRES which allows cap-independent translation. After addition of your desired protein you can express this system by inserting your favored promoter/terminator in the prefix/suffix your construct. For further information, please see under the subparts linked in the text.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 487
    Illegal BamHI site found at 569
    Illegal BamHI site found at 3432
    Illegal BamHI site found at 4862
    Illegal XhoI site found at 520
    Illegal XhoI site found at 539
    Illegal XhoI site found at 2674
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3849
    Illegal AgeI site found at 3196
    Illegal AgeI site found at 3551
    Illegal AgeI site found at 4626
    Illegal AgeI site found at 4981
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2059
    Illegal BsaI site found at 2465
    Illegal SapI site found at 736
    Illegal SapI.rc site found at 3151
    Illegal SapI.rc site found at 3629
    Illegal SapI.rc site found at 4170
    Illegal SapI.rc site found at 5059


[edit]
Categories
Parameters
None