Translational_Unit

Part:BBa_K1159113

Designed by: Dong-Jiunn Jeffery TRUONG   Group: iGEM13_TU-Munich   (2013-09-16)


Eukaryotic light-switchable TEV Protease translational unit (PhyB/PIF3 based)

This part encodes a light-switchable TEV Protease system consisting of two fusion proteins (BBa_K1159106 and BBa_K1159107) seperated by an polioviral IRES BBa_K1159300 which allows polycistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 487
    Illegal BamHI site found at 569
    Illegal BamHI site found at 3432
    Illegal XhoI site found at 520
    Illegal XhoI site found at 539
    Illegal XhoI site found at 2674
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3849
    Illegal AgeI site found at 3196
    Illegal AgeI site found at 3551
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2059
    Illegal BsaI site found at 2465
    Illegal SapI site found at 736
    Illegal SapI.rc site found at 3151
    Illegal SapI.rc site found at 3629
    Illegal SapI.rc site found at 4170


[edit]
Categories
Parameters
None