Designed by: Dong-Jiunn Jeffery TRUONG Group: iGEM13_TU-Munich (2013-09-14)
N-terminal Phytochrome Interacting Factor 3 (PIF3) in RFC
N-terminal Phytochrome Interacting Factor 3 (PIF3) is known to be recognized by Phytochrome B (PhyB) under red light exposition. This interaction between PhyB and PIF3 can be reverted by irradiation with far-red light. This part contains only the first 100 amino acid residues of PIF3 which is sufficient for light-dependent binding to PhyB. N- and C-terminal protein fusions seems not to affect the binding of PhyB. This part is flanked by RFC pre- and suffix for further protein fusions.
Nucleotide sequence in RFC 25, so ATGGCCGGC and ACCGGT were added (in italics) to the 5' and 3' ends: (underlined part encodes the protein) ATGGCCGGCCCTCTGTTT ... AATCATCATACCGGT ORF from nucleotide position -8 to 303 (excluding stop-codon)
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)