Part:BBa_K1159008

Secretory Catechol-2,3-dioxygenase (IgKappa-SigP_XylE) in RFC[25] N-Part

This part encodes for the enzyme catechol-2,3-dioxygenase (metapyrocatechase), which converts catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. This part is fused with a N-terminal signal peptide for secretion (Ig Kappa signal peptide from Mus musculus) with also works in moss (Physcomitrella patens). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0
Nucleotide sequence in RFC 25 N-Part using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein)  AACAATGGAAACC ... CCGGGCCATACCGGT  ORF from nucleotide position 5 to 106 (excluding stop-codon)
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below) 1  METDTLLLWVLLLWVPGSTGDTGNKGVMRPGHTG*
Sequence features: (with their position in the amino acid sequence, see the list of supported features) RFC25 scar (shown in bold):  19 to 20, 22 to 23
Amino acid composition: Ala (A) 0 (0.0%) Arg (R) 1 (2.9%) Asn (N) 1 (2.9%) Asp (D) 2 (5.9%) Cys (C) 0 (0.0%) Gln (Q) 0 (0.0%) Glu (E) 1 (2.9%) Gly (G) 6 (17.6%) His (H) 1 (2.9%) Ile (I) 0 (0.0%) Leu (L) 6 (17.6%) Lys (K) 1 (2.9%) Met (M) 2 (5.9%) Phe (F) 0 (0.0%) Pro (P) 2 (5.9%) Ser (S) 1 (2.9%) Thr (T) 5 (14.7%) Trp (W) 2 (5.9%) Tyr (Y) 0 (0.0%) Val (V) 3 (8.8%)
Amino acid counting Total number: 34 Positively charged (Arg+Lys): 2 (5.9%) Negatively charged (Asp+Glu): 3 (8.8%) Aromatic (Phe+His+Try+Tyr): 3 (8.8%)	Biochemical parameters Atomic composition: C163H259N43O48S2 Molecular mass [Da]: 3653.2 Theoretical pI: 5.36 Extinction coefficient at 280 nm [M-1 cm-1]: 11000 / 11000 (all Cys red/ox)
Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges
Codon usage Organism: E. coli B. subtilis S. cerevisiae A. thaliana P. patens Mammals Codon quality (CAI): good (0.68) good (0.68) acceptable (0.58) good (0.71) good (0.74) good (0.64)
Alignments (obtained from PredictProtein.org)    There were no alignments for this protein in the data base. The BLAST search was initialized and should be ready in a few hours.
Predictions (obtained from PredictProtein.org)
There were no predictions for this protein in the data base. The prediction was initialized and should be ready in a few hours.
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