Composite

Part:BBa_K1157006

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-10)

pRhl-RBS-mCherry-tt-Pc-RBS-RhlR-tt

This is a construct which expresses red fluorescence after the Rhl receptor binds with C4HSL (a kind of AHL). It acts as a biosensor of C4HSL in our project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 948
    Illegal NheI site found at 971
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1245
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1720


[edit]
Categories
Parameters
None