Composite

Part:BBa_K1156000

Designed by: Haylee Bachman   Group: iGEM13_GeorgiaTech   (2013-09-17)


RBS_T7_Pet(mCherry)

This is a composite part of an autodisplay technology translocating a fluorescent protein (mCherry). This part includes an T7 promoter with a lacI operator as well as an RBS(AGGA). The fluorescent protein will cleave post-translationally. This part can be used to quantify the surface density and activity of autodisplay technology. PET is a beta-barrel autodisplay technology native to E.coli. mCherry is a derivative of RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1826
    Illegal AgeI site found at 1700
    Illegal AgeI site found at 1793
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None