Coding
IGPD

Part:BBa_K1154002

Designed by: Adam Nighswander, Tanner Reeb and Bianca Maled   Group: iGEM13_RHIT   (2013-09-17)

Saccharomyces cerevisiae imidazoleglycerol-phosphate dehydratase (IGPD)

The Saccharomyces cerevisiae HIS3 gene encodes imidazoleglycerol-phosphate dehydratase (IGPD), which catalyzes the interconversion of D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate and 3-(imidazol-4-yl)-2-oxopropyl phosphate plus H2O. The dehydration reaction is a key step in the biosynthesis of histidine in many bacteria, fungi and plants. The gene is routinely used as a selectable marker in yeast histidine auxotrophs harboring a genomic his3 mutation (e.g., his3-Δ1). It also compliments Escherichia coli hisB mutations (e.g., ΔhisB720::kan).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 562
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None