Device
Part:BBa_K1150054
Designed by: Natalie Louis and Lisa Schmunk Group: iGEM13_Freiburg (2013-09-17)
Truncated SV40 dCas9 Device #3
Behind a strong SV40 promoter a HA-tag, a NLS and dCas9 followed by another NLS and a BGH terminator were assembled via the iGEM cloning method. The truncation of the huge dCas9 gene was performed by PCR over the backbone (=dCas9 in pSB1C3). The nucleotides between the primer pair is not amplified. One of the primers has an overlap compatible to the first bases of the other one, so that after this PCR a one fragment Gibson assembly could be performed.
Usage and Biology
This part can be used in the uniCAS Toolkit of team Freiburg as a shorter version of dCas9. Truncation 3: 1131bp of the anterior part of dCas9 are missing.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |