uniCAS RNAimer to EMX1
|RNAimer (EMX1 target)|
|Function||Targeting EMX1 with Cas9|
|RFC standard||RFC 25|
|Submitted by||Freiburg 2013|
This device derived from BBa_K1150034 can be used in combination with all devices with dCas9. It contains a DNA sequence coding for a crRNA that binds complementary to EMX1 target (GGAAGGGCCTGAGTCCGAGCAGAAGAAGAA).
For more information about dCas9 devices klick here.
Biology and Usage
Freiburg 2013 used this plasmid to test the efficiency of target EMX1 by activating or repressing a SEAP reporter gene.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 224
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 216