Composite

Part:BBa_K1149008

Designed by: Margarita Kopniczky   Group: iGEM13_Imperial_College   (2013-09-13)

Proteinase K (extracellular)

Encodes a peptidase from proteinase K family, breaks down polylactic acid. See here for construct with secretion tag.


References:

Larsen AN, Moe E, Helland R, Gjellesvik DR, Willassen NP.Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. FEBS J. 2006 Jan;273(1):47-60.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1009
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 356
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 210
    Illegal AgeI site found at 1531
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None