Composite
Part:BBa_K1148013
Designed by: Cécile Quéré Group: iGEM13_Bordeaux (2013-10-12)
4CL::STS (resveratrol synthase)
This part should allow resvertrol biosynthesis by the coupling of p-coumaric acid to CoA by the 4CL enzyme. Subsequently, coumaroyl-Co1 is converted into resveratrol by STS thanks to sequential addition of three malonyl-CoA units with the release of carbon dioxide. It is under the control of BBa_R0011 a lactose inducible promoter.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 320
Illegal EcoRI site found at 2465
Illegal EcoRI site found at 2748
Illegal PstI site found at 2280 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 320
Illegal EcoRI site found at 2465
Illegal EcoRI site found at 2748
Illegal PstI site found at 2280 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 320
Illegal EcoRI site found at 2465
Illegal EcoRI site found at 2748
Illegal BglII site found at 1125
Illegal BamHI site found at 194 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 320
Illegal EcoRI site found at 2465
Illegal EcoRI site found at 2748
Illegal PstI site found at 2280 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 320
Illegal EcoRI site found at 2465
Illegal EcoRI site found at 2748
Illegal PstI site found at 2280
Illegal AgeI site found at 982
Illegal AgeI site found at 1091
Illegal AgeI site found at 1217 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1088
Illegal SapI.rc site found at 2607
[edit]
Categories
Parameters
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