Composite

Part:BBa_K1148013

Designed by: Cécile Quéré   Group: iGEM13_Bordeaux   (2013-10-12)


4CL::STS (resveratrol synthase)

This part should allow resvertrol biosynthesis by the coupling of p-coumaric acid to CoA by the 4CL enzyme. Subsequently, coumaroyl-Co1 is converted into resveratrol by STS thanks to sequential addition of three malonyl-CoA units with the release of carbon dioxide. It is under the control of BBa_R0011 a lactose inducible promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 320
    Illegal EcoRI site found at 2465
    Illegal EcoRI site found at 2748
    Illegal PstI site found at 2280
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 320
    Illegal EcoRI site found at 2465
    Illegal EcoRI site found at 2748
    Illegal PstI site found at 2280
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 320
    Illegal EcoRI site found at 2465
    Illegal EcoRI site found at 2748
    Illegal BglII site found at 1125
    Illegal BamHI site found at 194
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 320
    Illegal EcoRI site found at 2465
    Illegal EcoRI site found at 2748
    Illegal PstI site found at 2280
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 320
    Illegal EcoRI site found at 2465
    Illegal EcoRI site found at 2748
    Illegal PstI site found at 2280
    Illegal AgeI site found at 982
    Illegal AgeI site found at 1091
    Illegal AgeI site found at 1217
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1088
    Illegal SapI.rc site found at 2607


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