Composite

Part:BBa_K1148001

Designed by: Cécile Quéré   Group: iGEM13_Bordeaux   (2013-10-12)


CrtB under PhlbA

PhlbA allows constitutive expression in L. bulgaricus. CrtB encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene (Misawa, N., Nakagawa, N., Kobayashi, K., Yamano, S., Nakamura, K., and Harashima, K. 1990. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. Journal of Bacteriology 172, 6704-612).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 134
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 134
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 134
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 134
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 134
    Illegal AgeI site found at 878
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None