Coding
CI-tag
Part:BBa_K1145007
Designed by: KUN LV Group: iGEM13_Nanjing-China (2013-09-14)
A protein which can inhibit the expression of Pλ.
CI is a protein which can inhibit the expression of Pλ, we use this part to regulate our whole system. It first comes from λ phage, and the length of its gene is 750bp. In our project, we modified this protein to make it better at inhibiting the expression of Pλ.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 226
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 226
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 226
Illegal BglII site found at 251 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 226
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 226
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |