Part:BBa_K1145002

A degrading enzyme which can degrade atrazine.

TrzN is a fantastic degrading enzyme which can degrade atrazine into a nontoxic chemical substance. It also comes from the genome of Arthrobacter aurescens. The length of its gene is 1371bp. With the help of TRM, this part can function much more efficiently.

This picture shows the atrazine concentration in supernatant of bacterial cultures, which were cultured for 24 hours in 37℃. Group 0 means no bacteria in the cultures; cultures in Group Wild Type contained the wild type K12 bacteria; cultures in Group Vector contained the strain K12 with pGFP; and cultures in Group TrzN+ contained the bacteria which are able to express TrzN. “*” means the difference between two groups which are connected by half square brackets is distinctive. From this chart we can conclude that TrzN is an efficient degrading enzyme in solving the problem of TrzN.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1317
  Illegal BamHI site found at 458
  Illegal XhoI site found at 229
  Illegal XhoI site found at 346
  Illegal XhoI site found at 1147
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 172