Part:BBa_K1144015
Prcna, hoxN
Prcnr,rcnR and PrcnA genes were used from E.coli regulation system.
Prcnr is a constitutive promoter, controlling the production of RcnR protein which
represses PrcnA. When there is nickel in the medium RcnR this induces a conformational
change in RcnR which prevents its binding to PrcnA, activating the production of the HoxN
protein in our construct. HoxN an efficient nickel membrane transporter form Cupriavidus
metallidurans that imports the metal into the cell. RFP was transcriptionally fused to HoxN
to monitor its expression.
Therefore, our system continuously detecting for nickel, and when this metal is detected,
it is absorbed.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 625
Illegal NgoMIV site found at 945 - 1000COMPATIBLE WITH RFC[1000]
References
Eitinger, T. Microbial Nickel Transport In Microbial Transport Systems. Ed. G. Winkelmann. Wiley-VCH 2001
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