Pbad-M13 on pSB3
M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin and a packaging sequence. The promoter upstream of g2p (gene 2 protein) is altered to bad promoter. A phage particle is formed only when the host cell receives arabinose because g2p is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 125
- 21Illegal BglII site found at 237
Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 6165
- 1000Illegal BsaI.rc site found at 136