Promoterless-M13-Plac-GFP on pSB3
We confirmed that M13 genome with two modifications related to our design kept plaque forming activity. One is replacement of the promoter for G2p (gene 2 protein) with a constitutive promoter, PlacIq (BBa_I14032). The other is accommodation of pSB3K3 backbone. Even though the plasmid has two different types of replication origins, M13 origin and pSB3 origin, this plasmid (BBa_K1139020) formed plaque. In contrast, construction intermediates without a promoter upstream of g2p coding sequence (Promoterless-M13 + Plac, Promoterless-M13 + Plac-GFP BBa_K1139022) could not form plaque.
For more information, see our work in Tokyo_Tech 2013 wiki.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 100
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 6026
- 1000Illegal BsaI.rc site found at 7287