Promoterless-M13 on pSB3
M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin and a packaging sequence. The promoter upstream of g2p is deleted. The capsid is formed only when g2p is expressed.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 99
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 6027
- 1000COMPATIBLE WITH RFC
|n/a||Promoterless-M13 on pSB3|