Plasmid_Backbone

Part:BBa_K1137006

Designed by: Matthew Deyell   Group: iGEM13_Paris_Bettencourt   (2013-10-04)

pSB6A3

pSB6A3 - contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with ColE1 replicon derived from pBR322 with a copy number of approximately 40 per cell. The cat gene has been replaced with ampR cassette conveying ampicillin resistance.

PB_PSB6A3.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 3544
    Illegal suffix found at 4635
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3544
    Illegal SpeI site found at 4636
    Illegal PstI site found at 4650
    Illegal NotI site found at 3550
    Illegal NotI site found at 4643
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3544
    Illegal XhoI site found at 3420
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3544
    Illegal suffix found at 4636
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3544
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3559
    Illegal SpeI site found at 4636
    Illegal PstI site found at 4650
    Illegal NgoMIV site found at 189
    Illegal AgeI site found at 4346
    Illegal AgeI site found at 4458
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 774
    Illegal SapI.rc site found at 2434


[edit]
Categories
Parameters
None