Composite

Part:BBa_K1136005

Designed by: Simin Jiang   Group: iGEM13_Nevada   (2013-09-16)


An L-Arabinose controlled endolysin from bacteriophage ΦKZ

L-arabinose induced KZ144 endolysin is a functional construct which contains the gene that encodes for the endolysin protein from the bacteriophage ΦKZ (BBa_K1136002) which targets Pseudomonas aeruginosa. The construct is under the control of an L-arabinose promoter (BBa_K206000) and the rrnb terminator (BBa_B0010). This part can be expressed in E. coli using 20% L-Arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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