Coding

Part:BBa_K1132015

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-20)


Cph8 (Cph1/EnvZ fusion) under TetR repressible promoter

Chimeric Cph1 light receptor/EnvZ protein requires phycocyanobilin (PCB) biosynthetic genes for PCB formation (ho1 and PcyA). The exposure to red light (660nm) inhibits the activity of the EnvZ histidine kinase domain. In the dark, the EnvZ histidine kinase phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpC promoter. This part must be used in E.coli deficient in wild-type EnvZ.

The promoter p(TetR) is constitutively on and repressed by TetR (Part:BBa_P0440). TetR binds to the p(TetR) and inhibits its operation. It regulates the light sensor Cph8. Tetracycline or aTc (anhydrotetracycline) binds to TetR and allows Cph8 production.

This part can be used with Part:BBa_K1132013 composed of the heme oxygenase (ho1), ferredoxin oxidoreductase (PcyA) and tetracycline repressor (TetR) under constitutive promoter. Both of these parts form a red light responsive system, under tetR regulation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 444
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None