Coding

Part:BBa_K1132013

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-20)

heme oxygenase (ho1), ferredoxin oxidoreductase (PcyA) and tetracycline repressor (TetR) under const

Two genes required for phycocyanobilin (PCB) biosynthesis and the coding region for the TetR protein with Ribosome Binding Site (RBS) under a strong promoter. The Part:BBa_K1132012 was inserted behind BBa_K608003, a strong promoter with a medium RBS.

ho1 produces biliverdin IXalpha, it represents the first of two steps in PCB biosynthesis. PcyA converts biliverdin IXalpha to PCB. PCB associates with EnvZ fusion protein, Cph8 (Part:BBa_I15010), to transduce a light signal into a genetic response.

TetR binds to the p(TetR) (Part:BBa_R0040) and inhibits its operation. It regulates the light sensor Cph8 (Part:BBa_K1132015). aTc (anhydrotetracycline) binds to TetR and allows Cph8 production. Both of these two parts construct the red light responsive system, under tetR regulation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1611
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1164
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None