Coding

Part:BBa_K1131005

Designed by: Ramya Prathuri   Group: iGEM13_Berkeley   (2013-09-13)


mutant oleD 'ASP' glucosyltransferase; S. antibiotics

This is the mutant version of oleD glucosyltransferase part BBa_K1131004. Mutations identified by Thorston et. al. 2010 at P67T, S132F, and A242V were introduced to oleD generating the 'ASP' version of the protein. OleD 'ASP' is reported to have a much larger set of substrates it can glucosylate compared to the non mutated oleD.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 4
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 702
    Illegal NgoMIV site found at 942
    Illegal NgoMIV site found at 1132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 401
    Illegal BsaI.rc site found at 667
    Illegal BsaI.rc site found at 1092


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Categories
Parameters
None