Coding

Part:BBa_K1131001

Designed by: Ramya Prathuri   Group: iGEM13_Berkeley   (2013-09-10)


Mutant Flavin-containing monooxygenase (FMO); M. aminisulfidivorans

As a corollary to the FMO from M. aminisulfidivorans submitted, this part has three specific mutations at F179A, G181A, F189A that eradicate FMO activity. This specific FMO converted indole to indoxyl which then oxidized to indigo, the well known dye. With these mutations, indigo was not observed, and was used as a control in several studies. This part contains the ORF of the mutated FMO only.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None