Composite

Part:BBa_K113021

Designed by: Wu Jingjing, Wang Jinyu   Group: iGEM08_HKUSTers   (2008-10-26)


Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter

T7 terminator + T7 polymerase + RBS + pBAD/araC + T7 terminator + GFP + RBS + tetR repressor + RBS +cI434 operator + T7 promoter (overlapping) T7 promoter + tetR operator + RBS + cI434 repressor + RBS + RFP + T7 terminator

The left side is in the antisense strand while the right side is in the sense strand.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4028
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2791
    Illegal BamHI site found at 3967
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3802
    Illegal AgeI site found at 7034
    Illegal AgeI site found at 7146
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4820
    Illegal SapI site found at 3784


[edit]
Categories
Parameters
None