Composite

Part:BBa_K113019

Designed by: Wu Jingjing, Wang Jinyu   Group: iGEM08_HKUSTers   (2008-10-26)


pBad/araC->T7 polymerase->GFP

pBad/araC is a promoter which can tightly control the expression of T7 polymerase. GFP following this construst is used to detect by microscope how efficient the promoter can work.

pBad/araC + RBS + T7 polymerse + RBS + GFP + T7 terminator

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3872
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4566
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None