Composite

Part:BBa_K113018

Designed by: Wu Jingjing, Wang Jinyu   Group: iGEM08_HKUSTers   (2008-10-26)


weaken overlapping T7 promoter->GFP/RFP

T7 terminator + GFP + RBS + T7 promoter (weaken overlapping) T7 promoter + RBS + RFP + T7 terminator

The left side of T7 promoter + RBS + GFP + T7T is in the antisense strand while the right side is in the sense strand. T7 promoters are overlapping only two base pairs while its 12th nucleotide is mutated from A to T.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1504
    Illegal AgeI site found at 1616
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 779


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Categories
Parameters
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