Composite

Part:BBa_K113017

Designed by: Wu Jingjing, Wang Jinyu   Group: iGEM08_HKUSTers   (2008-10-26)


more overlapping T7 promoter->GFP/RFP

T7 terminator + GFP + RBS + T7 promoter (more overlapping) T7 promoter + RBS + RFP + T7 terminator

The left side of T7 promoter + RBS + GFP + T7T is in the antisense strand while the right side is in the sense strand. T7 promoters are overlapping five base pairs with the third mutated from A to G.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1501
    Illegal AgeI site found at 1613
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 779


[edit]
Categories
Parameters
None