Composite

Part:BBa_K113015

Designed by: Wu Jingjing, Wang Jinyu   Group: iGEM08_HKUSTers   (2008-10-26)


bidirectional intact T7 promoters->GFP/RFP

T7 terminator + GFP + RBS + T7 promoter + T7 promoter + RBS + RFP + T7 terminator

The left side of T7 promoter + RBS + GFP + T7T is in the antisense strand while the right side is in the sense strand. So if there is only one copy of this construct, only one signal, either green or red will be turned on. We need to test the situation when T7 promoter of both sides is intact.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1518
    Illegal AgeI site found at 1630
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 779


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Categories
Parameters
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