Composite

Part:BBa_K113005

Designed by: Wang Jinyu   Group: iGEM08_HKUSTers   (2008-10-26)


pLac->tetR->pT7->tetR operator ->GFP

Whole construct of repressor test for right side. ---HKUSTers/2008

pLac + tetR + Terminator + pT7 + tetR operator + GFP + Terminator

The expression of tetR can be regulated by lac operon. When tetR is produced, it can bind to tetR operator so that the expression of GFP will be turned off. It is a test designed to check the effiency of the repressor tetR.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 931
    Illegal BsaI.rc site found at 1654


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Parameters
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