Reporter

Part:BBa_K113004

Designed by: WU Jingjing, Wang Jinyu   Group: iGEM08_HKUSTers   (2008-10-26)


pT7->tetR operator->GFP

tetR operator is inserted between T7 promoter and RBS in BBa_I719015, which is a T7 promoter-regulated GFP sequence. Thus tetR can repress the expression of GFP once the concentration of tetR reaches certain level.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 20
    Illegal BsaI.rc site found at 743


[edit]
Categories
Parameters
None