Composite

Part:BBa_K113002

Designed by: WU Jingjing, Wang Jinyu   Group: iGEM08_HKUSTers   (2008-10-23)

pLac->cI434->pT7->cI434 operator ->GFP

Whole construct of repressor test for left side. ---HKUSTers/2008

pLac + cI434 + Terminator + pT7 + cI434 operator + GFP + Terminator

The expression of cI434 can be regulated by lac operon. When cI434 is produced, it can bind to cI434 operator so that the expression of GFP will be turned off. It is a test designed to check the effiency of the repressor cI434.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 1604


[edit]
Categories
Parameters
None