Reporter

Part:BBa_K113001

Designed by: WU Jingjing, WANG Jinyu   Group: iGEM08_HKUSTers   (2008-10-23)

pT7->cI434 operator ->GFP

cI434 operator is inserted between T7 promoter and RBS in BBa_I719015, which is a T7 promoter-regulated GFP sequence. Thus cI434 can repress the expression of GFP once the concentration of cI434 reaches certain level.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 20
    Illegal BsaI.rc site found at 742


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Categories
Parameters
None