Plasmid

Part:BBa_K112952

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)


BBb1 assembly vector - C/A

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3198
    Illegal XbaI site found at 174
    Illegal PstI site found at 2157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3198
    Illegal NheI site found at 1016
    Illegal PstI site found at 2157
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 3198
    Illegal BamHI site found at 1
    Illegal XhoI site found at 2197
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3198
    Illegal XbaI site found at 174
    Illegal PstI site found at 2157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3198
    Illegal XbaI site found at 174
    Illegal PstI site found at 2157
    Illegal AgeI site found at 606
    Illegal AgeI site found at 930
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1298


[edit]
Categories
Parameters
chassis
copies
mcs
originp15A
resistanceAC