Part:BBa_K1128007
Methyl CpG Binding Protein 2 MECP2)(
Methyl CpG binding protein 2 (MECP2) is a mammalian protein that binds to methylated CpG sites by recruiting histone deacetylases, and is essential for embryonic development. The Penn iGEM team is using this protein to test the feasibility of transcriptional silencing using CpG methylation in bacteria using a cell-free assay and a methylated red fluorescent protein gene. The possibility of transcriptional silencing in E. Coli using methylation that is completely orthogonal to native E. Coli methylation can open up whole new for synthetic biologists, giving them a layer of control on genetic systems that has yet to be touched by traditional synthetic biology. This part can be purified for use in cell-free assays, and used for CpG methylated transcriptional silencing studies.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 158
Illegal PstI site found at 836 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 158
Illegal PstI site found at 836 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 158
Illegal PstI site found at 836 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 158
Illegal PstI site found at 836 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 591
Illegal SapI.rc site found at 642
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