Coding

Part:BBa_K1128002

Designed by: Daniel Cabrera   Group: iGEM13_Penn   (2013-09-18)

M.SssI with a linker - ready for fusing

For full characterization see BBa_K1128000 and BBa_K1128001. This is an M.SssI methyltransferase protein with a linker on the N terminus. The part is ready for fusion to transcription factors. The linker is composed of a string of glycine residues.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 568
    Illegal BsaI.rc site found at 449
    Illegal SapI site found at 652


[edit]
Categories
Parameters
None