Coding

Part:BBa_K112624

Designed by: Sherine Cheung   Group: iGEM08_UC_Berkeley   (2008-10-22)
{Pbad}.{rbs_pelB>}.{} in BBb

{Pbad}.{rbs_pelB>}.{<phoA>}

This part is assembled with pBad promoter, rbs.pelB> (pelB prepro with native rbs and start codon, but no stop), and a <phoA> region (no start or stop codon).

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1317
    Illegal NgoMIV site found at 1540
    Illegal NgoMIV site found at 1987
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1005


[edit]
Categories
Parameters
None