RNA

Part:BBa_K1126008

Designed by: Keita Kinose   Group: iGEM13_Kyoto   (2013-09-13)


Fusion1 antisense

Fusion1 antisense is the functional RNA which regulates the transcription of downstream gene of Fusion1 attenuator (BBa_K1126005).

In the absence of antisense RNA, secondary structure of Fusion1 attenuator locus does not affect transcription. However, when mRNA of this locus is partly coupled complementary with mRNA of Fusion1 antisense, this locus is folded into Rho-independent terminator and terminates the transcription right before the gene. Downstream gene is somewhat silenced by this mechanism (the efficiency is shown to be 24% in Escherichia coli).

This mechanism is originally found in the regulation system of plasmid pT181 in gram negative bacteria Staphylococcus aureus, and Fusion1 antisence is made by arranging the sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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