RNA

Part:BBa_K1126006

Designed by: Keita Kinose   Group: iGEM13_Kyoto   (2013-09-13)


pT181 antisense

pT181 antisense is the functional RNA which regulates the transcription of downstream gene of pT181 attenuator (BBa_K1126003).

In the absence of pT181 antisense, secondary structure of pT181 attenuator locus does not affect transcription. However, when mRNA of this locus is partly coupled complementary with pT181 antisense RNA, this locus is folded into Rho-independent terminator and terminates the transcription right before the gene. The efficiency of this termination is very high and downstream gene is strongly silenced (the efficiency is shown to be 84% in Escherichia coli).

This mechanism is originally found in the regulation system of plasmid pT181 in gram negative bacteria Staphylococcus aureus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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