Part:BBa_K1126004

Fusion3m2 attenuator

Fusion3m2 attenuator is the locus where regulates the transcription of downstream gene with Fusion 6 antisense (BBa_K1126007) if it is located between promoter and ribosome binding site.

In the absence of antisense RNA, secondary structure of Fusion3m2 attenuator locus does not affect transcription. However, when mRNA of this locus is partly coupled complementary with mRNA of Fusion6 antisense, this locus is folded into Rho-independent terminator and terminates the transcription right before the gene. Downstream gene is somewhat silenced by this mechanism (the efficiency is shown to be 44% in Escherichia coli).

This mechanism is originally found in the regulation system of plasmid pT181 in gram negative bacteria Staphylococcus aureus, and Fusion3m2 attenuator is made by arranging the sequence.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]