Composite

Part:BBa_K1124213

Designed by: Takahiro Yamada   Group: iGEM13_UT-Tokyo   (2013-09-15)

Pconst (strong)-luxR-Pconst (strong)-amilGFP-LVA

The construct for Multicellular Analog Clock. This BBa_K1124213 contains a strong constitutive promoer and LuxR and amilGFP. 

 LuxI and LuxR forms the positive feedback loop of AHL production.

You can make the full construct of the Multicellular Analog Clock with BBa_K1124100. The excition wave of gene expression are generated on an agar plate and visualized for naked eyes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 994
    Illegal NheI site found at 1017
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None