Composite

Part:BBa_K1124212

Designed by: Takahiro Yamada   Group: iGEM13_UT-Tokyo   (2013-09-15)

Pconst (strong)-luxR

The construct for Multicellular Analog Clock. This BBa_K1124212 contains a strong constitutive promoer and LuxR. 

 LuxI and LuxR forms the positive feedback loop of AHL production.
 amilGFP is the yellow reporter protein for visualization of background.

You can make the full construct of the Multicellular Analog Clock with BBa_K1124100. The excition wave of gene expression are generated on an agar plate and visualized for naked eyes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None