Composite

Part:BBa_K1124210

Designed by: Takahiro Yamada   Group: iGEM13_UT-Tokyo   (2013-09-15)

pLux-mCherry-aiiA

The construct for Multicellular Analog Clock. This BBa_K1124210 contains positive feedback no tetR system.

 AiiA is the suppressor for negative feedback loops.
 pLac is only controlled by LuxR and AHL.
 mCherry is the red reporter protein for visualization of the excitation wave (gclock handh).

You can make the full construct of the Multicellular Analog Clock with BBa_K1124100. The excition wave of gene expression are generated on an agar plate and visualized for naked eyes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 954
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None