Composite
Part:BBa_K1124210
Designed by: Takahiro Yamada Group: iGEM13_UT-Tokyo (2013-09-15)
pLux-mCherry-aiiA
The construct for Multicellular Analog Clock. This BBa_K1124210 contains positive feedback no tetR system.
AiiA is the suppressor for negative feedback loops. pLac is only controlled by LuxR and AHL. mCherry is the red reporter protein for visualization of the excitation wave (gclock handh).
You can make the full construct of the Multicellular Analog Clock with BBa_K1124100. The excition wave of gene expression are generated on an agar plate and visualized for naked eyes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 954
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |