Composite
Part:BBa_K1124209
Designed by: Takahiro Yamada Group: iGEM13_UT-Tokyo (2013-09-15)
pLux-mCherry-luxI
The construct for Multicellular Analog Clock. This BBa_K1124209 contains positive feedback no tetR system.
LuxI and LuxR forms the positive feedback loop of AHL production. mCherry is the red reporter protein for visualization of the excitation wave (gclock handh). pLac is only controlled by LuxR and AHL
You can make the full construct of the Multicellular Analog Clock with BBa_K1124100. The excition wave of gene expression are generated on an agar plate and visualized for naked eyes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1491
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |