Generator

Part:BBa_K1124110

Designed by: Takahiro Yamada   Group: iGEM13_UT-Tokyo   (2013-09-15)

amilGFP(+LVA) generator(+RBS, +terminator)


Usage and Biology

This parts express amilGFP(+LVA).

The protein amilGFP is very useful yellow chromoprotein and is used as reporter protein.

This parts is amilGFP with degradation tag(LVA tag) which increase decay rate by PCR using the primers.

This rapid degradation allows real-time imaging.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None