Composite

Part:BBa_K1124103

Designed by: Takahiro Yamada   Group: iGEM13_UT-Tokyo   (2013-09-15)

clock device (plux/tet) (-luxI, +tetR, +aiiA, ++sRNA, +amilGFP)(negative feedback only)

The full construct for Multicellular Analog Clock. This BBa_K1124103 contains only negative feedback. You can check the negative feedback loop of the clock "gear". 

 TetR, AiiA, and sRNAs are the suppressors for negative feedback loops.
 mCherry is the red reporter protein for visualization of the excitation wave (gclock handh).
 amilGFP is the yellow reporter protein expressed constitutively as an internal standard.

You can make the full construct of the Multicellular Analog Clock with BBa_K1124100. The excition wave of gene expression are generated on an agar plate and visualized for naked eyes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3204
    Illegal NheI site found at 3227
    Illegal NheI site found at 4191
    Illegal NheI site found at 4214
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2337
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 326
    Illegal BsaI.rc site found at 654


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